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Catalog Number | orb1088127 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein(Bax). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Associated X Protein(Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein(Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein(Bax) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Sheep |
Range | 0.32-20 ng/mL |
Sensitivity | 0.124 ng/mL |
Protein Length | 3.5h |
Alternative names | BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-l Read more... |
Note | For research use only |
Sample Types | Tissue homogenates, cell lysates and other biological fluids. |
Expiration Date | Please enquire. |
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