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Catalog Number | orb1259566 |
---|---|
Category | Antibodies |
Description | SHB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human SHB (NP_003019.2). |
Concentration | batch dependent |
Dilution range | WB: 1:200 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 55kDa |
Target | SHB |
UniProt ID | Q15464 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | bA3J10.2, SH2 domain-containing adapter protein B, Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SHB antibody (orb1259566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
ELISA, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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