SH3GLB1 Antibody

• Catalog Number: orb1247894
• Category: Antibodies
• Description: SH3GLB1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QKGKVPITYLELLN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SH3GLB1
• UniProt ID: Q9Y371
• NCBI: NP_057093.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247894 staining (0.3 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247894 (2.5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.