SH2B3 Antibody

• Catalog Number: orb1247304
• Category: Antibodies
• Description: SH2B3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: NGPALQPSSPC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in lysates of cell line MOLT4 (calculated MW of 63.2kDa according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1.0 ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SH2B3
• UniProt ID: Q9UQQ2
• NCBI: NP_005466.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SH2B3, LNK, SH2B adaptor protein 3, lymphocyte ada
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in lysates of cell line MOLT4 (calculated MW of 63.2kDa according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1.0 ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247304 (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SH2B3 and probed with orb1247304 (mock transfection in first lane).