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SH2B3 Antibody (N-Term)
Catalog Number: orb1424579
| Catalog Number | orb1424579 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to SH2B3. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB57762 |
| Tested applications | FC, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | Synthesized Peptide |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, FC: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 63225 |
| Target | SH2B3 |
| UniProt ID | Q9UQQ2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | SH2B adapter protein 3, Lymphocyte adapter protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-SH2B3 Antibody (N-Term) at 1:2000 dilution + U-251 MG whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
