SGLT2/SLC5A2 Antibody

• Catalog Number: orb546342
• Category: Antibodies
• Description: SGLT2/SLC5A2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human SGLT2/SLC5A2 recombinant protein (Position: A15-N656).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 73 kDa
• UniProt ID: P31639
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Sodium/glucose cotransporter 2; Na (+)/glucose cot
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-SLC5A2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SLC5A2 using anti-SLC5A2 antibody.Lane 1:human HL-60 cell;2:human THP-1 cell;3:rat kidney tissue;4:rat spleen tissue;5:rat lung tissue;6:mouse kidney tissue;7:mouse spleen tissue;8:mouse lung tissue.

WB analysis of SLC5A2 using anti-SLC5A2 antibody.Lane 1:human HL-60 cell; 2:human THP-1 cell.

WB analysis of SLC5A2 using anti-SLC5A2 antibody.Lane 1:rat kidney tissue; 2:rat spleen tissue; 3:rat lung tissue; 4:mouse kidney tissue; 5:mouse spleen tissue; 6:mouse lung tissue.

IHC analysis of SLC5A2 using anti-SLC5A2 antibody.SLC5A2 was detected in paraffin-embedded section of human renal cancer tissues.

IHC analysis of SLC5A2 using anti-SLC5A2 antibody.SLC5A2 was detected in paraffin-embedded section of mouse kidney tissues.