SGK223 antibody

• Catalog Number: orb215993
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SGK223 (Phospho-Y413)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y413 of human SGK223 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SGK223
• Entrez: 157285
• UniProt ID: Q86YV5
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Tyrosine-protein kinase SgK223 antibody, anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SGK223 (Phospho-Y413) expression in U251MG (A), HeLa (B) whole cell lysates. (Predicted band size: 149 kD; Observed band size: 175 kD)

Immunohistochemical analysis of SGK223 (Phospho-Y413) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.