SFTPC antibody

• Catalog Number: orb1438554
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SFTPC.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31998
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:25, WB: 1:2000, FC: 1:25, IHC-P: 1:200
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 21053
• Target: SFTPC
• UniProt ID: P11686
• NCBI: NP_001165828.1, NP_001165881.1, NP_003009.2
• Alternative names: Pulmonary surfactant-associated protein C, SP-C, P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of A549 cells labeling SFTPC with orb1438554 at 1:25 dilution, followed by Dylight®488-conjugated goat anti-Rabbit IgG(green).Cytoplasmic actin detected with Dylight® 554 Phalloidin(red).Nuclear counter stain is DAPI(blue).

Western blot:All lanes:Anti-SFTPC Antibody (N-term) at 1:2000 dilution.Lane 1:Human lung lysate;Lane 2:Mouse lung lysate;Lane 3:Rat lung lysate.

Flow cytometric analysis of A549 cells stained with orb1438554(green line) at 1:25 dilution.Secondary antibody was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed;Isotype control antibody (blue line) was rabbit IgG1.

Immunohistochemical analysis of Human lung section using SFTPC Antibody at 1:200 dilution.A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.