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Catalog Number | orb2292191 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant SFRS10. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7A1 |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | SFRS10 (NP_004584, 120 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | LGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRP |
NCBI | NP_004584 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged SFRS10 is approximately 0.1 ng/ml as a capture antibody.
Western Blot analysis of SFRS10 expression in transfected 293T cell line by SFRS10 monoclonal antibody (M01), clone 7A1. Lane 1: SFRS10 transfected lysate (33.7 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of SFRS10 over-expressed 293 cell line, cotransfected with SFRS10 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SFRS10 monoclonal antibody (M01), clone 7A1 (Cat # orb2292191). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (34.54 KDa).