SFRP4 Antibody

• Catalog Number: orb334621
• Category: Antibodies
• Description: SFRP4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human SFRP4 recombinant protein (Position: A22-K303). Human SFRP4 shares 96.8% and 96.1% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA , 0.1-0.5μg/ml, Human, -Western blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 39827 MW
• UniProt ID: Q6FHJ7
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Secreted frizzled-related protein 4;sFRP-4;Frizzle
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SFRP4 using anti-SFRP4 antibody.Lane 1:A549 cell;2:SW620 cell.

IHC analysis of SFRP4 using anti-SFRP4 antibody. SFRP4 was detected in a paraffin-embedded section of human endometrial carcinoma tissue.