SFRP2 Antibody

• Catalog Number: orb614136
• Category: Antibodies
• Description: SFRP2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence of human SFRP2 (KFLCSLFAPVCLDDLDETIQPCHSLCVQ).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: "Western blot, 0.1-0.25μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human"
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 38 kDa
• UniProt ID: Q96HF1
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Secreted frizzled-related protein 2; FRP-2; sFRP-2
• Note: For research use only
• Application notes: "Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-SFRP2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of CACO-2 cells using anti-SFRP2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IF analysis of SFRP2 using anti-SFRP2 antibody.

WB analysis using anti-SFRP2 antibody.Lane 1:HeLa cell, Lane 2:A431 cell, Lane 3:Caco-2 cell, Lane 4:U-87MG cell.