SF3B4 Antibody

• Catalog Number: orb1523875
• Category: Antibodies
• Description: SF3B4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 374 and 424
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SF3B4
• UniProt ID: Q15427
• NCBI: NP_005841.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SF3b50; spliceosome-associated protein (U2 snRNP);
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse SF3B4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , Jurkat (50µg) , and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody orb1523875 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human SF3B4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-SF3B4 antibody orb1523875 used for IP at 6 µg/mg lysate.

Detection of mouse SF3B4 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SF3B4 (Cat. No. orb1523875) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human SF3B4 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SF3B4 (Cat. No. orb1523875) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB