SF2/SRSF1 Antibody

• Catalog Number: orb251587
• Category: Antibodies
• Description: SF2/SRSF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2 (6-33aa VIRGPAGNNDCRIYVGNLPPDIRTKDIE), identical to the related mouse sequence.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 27745 MW
• UniProt ID: Q07955
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serine/arginine-rich splicing factor 1;Alternative
• Note: For research use only
• Application notes: WB: The detection limit for SF2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody.Lane 1:human HeLa cell; 2:human SW620 cell; 3:human MCF-7 cell; 4:human 293T cell; 5:rat brain tissue; 6:rat liver tissue; 7:mouse brain tissue; 8:mouse liver tissue.

IHC analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody. SF2/SRSF1 was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody. SF2/SRSF1 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody. SF2/SRSF1 was detected in a paraffin-embedded section of rat brain tissue.