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Catalog Number | orb1274406 |
---|---|
Category | Antibodies |
Description | SETD7 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 41kDa |
Target | SETD7 |
UniProt ID | Q8WTS6 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | SETD7, FLJ21193, KIAA1717, KMT7, SET7, SET7/9, SET Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SETD7 antibody (orb1274406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and SETD7 knockout (KO) HeLa cells, using SETD7 antibody (orb1274406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
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