Serum Response Factor Antibody / SRF

• Catalog Number: orb2634819
• Category: Antibodies
• Description: Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-SRF-1F7
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Serum Response Factor antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Serum Response Factor antibody is available for research use only.
• UniProt ID: P11831
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Serum Response Factor antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with Serum Response Factor antibody (clone PCRP-SF7-1F1) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human HeLa cells using Serum Response Factor antibody (green, clone PCRP-SRF-1F7) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with Serum Response Factor antibody (blue, clone PCRP-SRF-1F7) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free Serum Response Factor antibody (clone PCRP-SRF-1F7) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Serum Response Factor antibody (clone PCRP-SRF-1F7). These results demonstrate the foremost specificity of the PCRP-SRF-1F7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.