SERPINE1 Antibody

• Catalog Number: orb1247895
• Category: Antibodies
• Description: SERPINE1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-GFKIDDKGMAPALRH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Liver and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6ug/ml. A customer successfully used this product on Human Breast cancer.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SERPINE1
• UniProt ID: P05121
• NCBI: NP_000593.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cys
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Liver and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6ug/ml. A customer successfully used this product on Human Breast cancer.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
• Expiration Date: 12 months from date of receipt.

orb1247895 (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247895 (1.5 ug/ml) as the reporter antibody.

orb1247895 (4 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

orb1247895 (0.1 ug/ml) staining of A549 (A), HepG2 (B) and HeLa (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.