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Catalog Number | orb259615 |
---|---|
Category | Antibodies |
Description | Serotonin transporter/SLC6A4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4 (57-85aa DDTRHSIPATTTTLVAELHQGERETWGKK), different from the related mouse and rat sequences by six amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 70325 MW |
UniProt ID | P31645 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Sodium-dependent serotonin transporter;5HT transpo Read more... |
Note | For research use only |
Application notes | WB: The detection limit for SLC6A4 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of A549(Lane 1), MM231(Lane 2), HELA(Lane 3), 22RV1(Lane 4) Whole Cell Lysate at 40ug using SLC6A4 antibody
Immunohistochemical staining of Human Mammary Cancer Tissue using SLC6A4 antibody
Immunohistochemical staining of Rat Brain Tissue using SLC6A4 antibody
IHC, WB | |
Hamster | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Hamster | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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