Sepharose Protein G

SKU: orb348763

Description

Sepharose Protein G

Images & Validation

• Tested Applications: IP
• Application Notes:
  Protein G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Key Properties

• Biological Activity: greater than 20mg/ml human IgG bound per CC Sepharose
• Purity: Sepharose Protein G binds to all IgG subclasses from human, monkey, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
• Conjugation: Sepharose

Storage & Handling

• Storage: Store SEPHAROSE PROTEIN G at 4° C prior to opening. DO NOT FREEZE. Supplied in 20% Ethanol/Water
• Form/Appearance: Suspension of agarose beads
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

ProG, Streptococcus G protein, Sepharose Protein G

Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab')2 region.

Net1 deletion inhibits PyMT-dependent ERK1/2 and Akt1 activation. d Western blot analysis of proteins coimmunoprecipitating with PyMT from tumors of genotypes shown. Representative experiment from four independent experiments. Net1 neuroepithelial transforming gene 1, PP2A protein phosphatase 2A, PyMT polyoma middle T antigen, Shc Src homology domain 2 containing, Src SRC proto-oncogene, non-receptor tyrosine kinase.

Quick Database Links

UniProt

P19909