You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Sepharose Protein G
Catalog Number: orb348763
| Catalog Number | orb348763 |
|---|---|
| Category | Proteins |
| Description | Sepharose Protein G |
| Conjugation | Sepharose |
| Form/Appearance | Suspension of agarose beads |
| Purity | Sepharose Protein G binds to all IgG subclasses from human, monkey, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. |
| UniProt ID | P19909 |
| Tested applications | IP |
| Dilution range | IP: User Optimized |
| Application notes | Protein G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction. |
| Biological Activity | greater than 20mg/ml human IgG bound per CC Sepharose |
| Storage | Store SEPHAROSE PROTEIN G at 4° C prior to opening. DO NOT FREEZE. Supplied in 20% Ethanol/Water |
| Alternative names | ProG, Streptococcus G protein, Sepharose Protein G |
| Hazard Information | Non-Toxic |
| Note | For research use only |
Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab')2 region.
Net1 deletion inhibits PyMT-dependent ERK1/2 and Akt1 activation. d Western blot analysis of proteins coimmunoprecipitating with PyMT from tumors of genotypes shown. Representative experiment from four independent experiments. Net1 neuroepithelial transforming gene 1, PP2A protein phosphatase 2A, PyMT polyoma middle T antigen, Shc Src homology domain 2 containing, Src SRC proto-oncogene, non-receptor tyrosine kinase.
- Item 1 of 3
Sepharose Protein A/G [orb348756]
IP, WB
Sepharose
The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies.
2 ml
