Sepharose Protein A

SKU: orb348755

Description

Sepharose Protein A

Images & Validation

• Application Notes:
  Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Key Properties

• Purity: Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
• Conjugation: Sepharose

Storage & Handling

• Storage: Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE.
• Form/Appearance: Suspension of agarose beads
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 20% (v/v) Ethanol
• Concentration: 6.0 mg/cc
• Expiration Date: 6 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

ProA, Staphylococcus A protein, Sepharose Protein A

Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab')2 region.

Quick Database Links

UniProt

P38507