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Sepharose Protein A
Catalog Number: orb348754
| Catalog Number | orb348754 |
|---|---|
| Category | Proteins |
| Description | Sepharose Protein A |
| Tested applications | IP |
| Concentration | 6.0 mg/cc |
| Dilution range | IP: User Optimized |
| Form/Appearance | Suspension of agarose beads |
| Purity | Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies. |
| Conjugation | Sepharose |
| Hazard Information | Non-Toxic |
| UniProt ID | P38507 |
| Storage | Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE. |
| Buffer/Preservatives | None |
| Alternative names | ProA, Staphylococcus A protein, Sepharose Protein Read more... |
| Note | For research use only |
| Application notes | Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction. |
| Expiration Date | 6 months from date of receipt. |
Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab')2 region.
- Item 1 of 4
Sepharose Protein A [orb348753]
IP
Sepharose
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
2 ml - Item 1 of 3
Sepharose Protein A/G [orb348756]
IP, WB
Sepharose
The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies.
2 ml - Item 1 of 1
Sepharose Protein A [orb348755]
IP
Sepharose
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
25 ml
