Semaphorin 3B/SEMA3B Antibody

• Catalog Number: orb546368
• Category: Antibodies
• Description: Semaphorin 3B/SEMA3B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 83 kDa
• UniProt ID: Q13214
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Semaphorin-3B; Sema A (V); Semaphorin-V; Sema V; S
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-SEMA3B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SEMA3B using anti-SEMA3B antibody.Lane 1:human placenta tissue;2:human U2OS cell;3:human A549 cell;4:human U-87MG cell.

WB analysis of SEMA3B using anti-SEMA3B antibody.Lane 1:rat thymus tissues lysates2:rat kidney tissue;3:mouse thymus tissue;4:mouse lung tissue;5:mouse small intestine tissue;6:mouse kidney tissue;7:mouse HEPA1-6 cell.

IF analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in immunocytochemical section of A431 cells.

IHC analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of SEMA3B using anti-SEMA3B antibody. SEMA3B was detected in paraffin-embedded section of rat brain tissue.