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Catalog Number | orb1960161 |
---|---|
Category | Proteins |
Description | SEMA7A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 69.5 kDa and the accession number is O75326-1. |
Tag | C-His |
Purity | > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
MW | 69.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result. |
UniProt ID | O75326 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | SEMA7A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 69.5 kDa and the accession number is O75326-1. |
Expression Region | Gln45-Ala648 |
Storage | -20°C |
Note | For research use only |
Application notes | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 69.2 kDa after removal of the signal peptide. The apparent molecular mass of SEMA7A-His is approximately 70-100 kDa due to glycosylation. | |
E.coli |