SEMA5A antibody

• Catalog Number: orb19272
• Category: Antibodies
• Description: Goat polyclonal antibody to SEMA5A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.03 μg/ml, IHC-P: 4-6 μg/ml
• Conjugation: Unconjugated
• MW: 121
• Target: Semaphorin 5A
• Entrez: 9037
• Protein Sequence: DEATKKACYSKGKS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SEMA5A antibody, anti sema domain, seven thro
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Kidney using SEMA5A antibody