SEMA3E antibody

• Catalog Number: orb19386
• Category: Antibodies
• Description: Goat polyclonal antibody to SEMA3E
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.03 μg/ml, IHC-P: 4-6µg/ml
• Conjugation: Unconjugated
• MW: 89.2; 82.4
• Target: Semaphorin 3E (internal)
• Entrez: 9723
• Protein Sequence: NGGRYGTTKDYPDD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SEMA3E antibody, anti sema domain, immunoglob
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Prostate using SEMA3E antibody