Selenophosphate Synthetase 1 Antibody

• Catalog Number: orb750515
• Category: Antibodies
• Description: Sephs1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Mouse
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.
• Antibody Type: Primary Antibody
• Concentration: 85 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: This antibody is directed against mouse SPS1. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with SPS1 from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q8BH69
• NCBI: AAH66037.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Selenophosphate Synthetase 1 Antibody,
• Note: For research use only
• Application notes: This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell (lane 1), mouse kidney, and liver tissue lysates (lanes 3-4). No signal is seen in NIH3T3 cells after pre-treatment with SPS1 siRNA (lane 2). SPS1 control (lane 5-6). Negligible cross-reactivity is seen against recombinant SPS2 (lane 7). The primary antibody was used at a 1:1000 dilution.