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Catalog Number | orb413075 |
---|---|
Category | Antibodies |
Description | SCRIBBLE Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 240 kDa |
UniProt ID | Q14160 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein scribble homolog; Scribble; hScrib; Protei Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-SCR1B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A549 cells using anti-SCR1B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of SCRIBBLE using anti-SCRIBBLE antibody.Lane 1:human MCF-7 cell;2:human COLO-320 cell;3:human 22RV1 cell;4:human SGC-7901 cell.
IF analysis of SCRIBBLE using anti-SCRIBBLE antibody. SCRIBBLE was detected in immunocytochemical section of U20S cells.
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