SCIMP antibody

• Catalog Number: orb154406
• Category: Antibodies
• Description: Mouse monoclonal antibody to SCIMP which also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse.
• Clonality: Monoclonal
• Clone Number: NVL-07
• Tested applications: FC, ICC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant intracellular part of human SCIMP
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: SCIMP
• Entrez: 388325
• UniProt ID: Q6UWF3
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-NVL antibody
• Note: For research use only
• Application notes: Flow cytometry: Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (intracellular staining) of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.

Western blotting analysis of human SCIMP using mouse monoclonal antibody NVL-07 on lysates of human SCIMP transfectants under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SCIMP was detected around 17-22 kDa.