SATB1 antibody

• Catalog Number: orb20363
• Category: Antibodies
• Description: Goat polyclonal antibody to SATB1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 86.0. 89.0; 78.3
• Target: SATB1
• Entrez: 6304
• Protein Sequence: ESDEENRQKTRPRT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SATB1 antibody, anti SATB homeobox 1 antibody
• Note: For research use only
• Application notes: IHC: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration, 3-5μg/ml.WB: Experiments gave bands at approx. 110kDa and 85kDa in lysates of cell line MOLT4 after 0.3 μg/ml antibody staining. The observed 110kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol. 1998 Apr 20;141(2):335-48.; PMID: 9548713). This protein has a calculated MW of 86.0kDa according to NP_002962.1. Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MOLT4 lysate (35µg protein in RIPA buffer) using SATB1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human thymus tissue using SATB1 antibody