SARS S protein (R667A, K968P, V969P)

• Catalog Number: orb750333
• Category: Proteins
• Description: The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Reactivity: Virus
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 136.3 kDa
• Target: Spike protein
• Protein Sequence: AAP13567.1 (R667A, K968P, V969P)
• Source: SARS S protein (R667A, K968P, V969P), His Tag (orb750333) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.
• Expression System: HEK293
• Expression Region: Ser 14 - Pro 1195
• Endotoxins: 1.0 EU per μg
• NCBI: K968, V969, AAP13567.1, R667
• Storage: -20℃
• Buffer/Preservatives: PBS
• Alternative names: Spike,S protein,Spike glycoprotein,S glycoprotein
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.