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Catalog Number | orb1953651 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 Spike S1+S2 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R & D950N, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 136.01 kDa and the accession number is A0A6G7K2L4. |
Tag | C-His-Avi |
Purity | 98.00% |
UniProt ID | A0A6G7K2L4 |
MW | 136.01 kDa (predicted) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | Baculovirus Insect Cells |
Biological Origin | SARS-CoV-2 |
Biological Activity | SARS-CoV-2 Spike S1+S2 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R & D950N, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 136.01 kDa and the accession number is A0A6G7K2L4. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations 157-158 deletion, T19R, G142D, E156G, L452R, T478K, D614G, P681R, D950N) (Met1-Pro1213) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as B.1.617.2) which emerged in the India. Predicted N terminal: Val 16 |
Storage | -20°C |
Note | For research use only |