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Catalog Number | orb1953646 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 Spike S1 Protein (T95I & G142D & E154K & L452R & E484Q & D614G & P681R, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 78.39 kDa and the accession number is A0A6G7K2L4. |
Tag | C-His-Avi |
Purity | 98.00% |
MW | 78.39 kDa (predicted); 110.06 kDa (reducing condition, due to glycosylation) |
UniProt ID | A0A6G7K2L4 |
Expression System | HEK293 Cells |
Biological Origin | SARS-CoV-2 |
Biological Activity | SARS-CoV-2 Spike S1 Protein (T95I & G142D & E154K & L452R & E484Q & D614G & P681R, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 78.39 kDa and the accession number is A0A6G7K2L4. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations T95I, G142D, E154K, L452R, E484Q, D614G, P681R) (Met1-Arg685) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as B.1.617.1) which emerged in the India. Predicted N terminal: Val 16 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |