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Catalog Number | orb1953804 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 Spike RBD Protein (N501Y, hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 52 kDa and the accession number is A0A6G7K2L4. |
Tag | C-Fc |
Purity | 98.00% |
UniProt ID | A0A6G7K2L4 |
MW | 52 kDa (predicted); 57 kDa (reducing condition, due to glycosylation) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | SARS-CoV-2 |
Biological Activity | SARS-CoV-2 Spike RBD Protein (N501Y, hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 52 kDa and the accession number is A0A6G7K2L4. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with the Fc region of human IgG1 at the C-terminus. The mutation were identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom. Predicted N terminal: Ser |
Storage | -20°C |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of RBD(N501Y)-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of RBD(K417N, E484K,N501Y)-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by SDS-PAGE | |
150-180 kDa |
98.00% | |
51.84 kDa (predicted); 56.07 kDa (reducing condition, due to glycosylation) |
98.00% | |
51.94 kDa (predicted); 56.01 kDa (reducing condition, due to glycosylation) |