SARS-CoV-2 Omicron BA.5 Variant Pseudovirus

• Catalog Number: orb1671376
• Category: Small Molecules
• Description: SARS-CoV-2 Omicron BA.5 Variant Pseudovirus
• Tested applications: NeA
• Form/Appearance: Liquid
• Target: S
• UniProt ID: P0DTC1
• Protein Sequence: Omicron Variant Spike BA.5 Met 1 - Cys 1251 (T19I, L24S, 25-27del, 69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K)
• Source: HEK293 cells
• NCBI: QHD43416
• Storage: The pseudovirus is stable in a liquid state at -70˚ for 12 months. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: DMEM/10% FBS
• Alternative names: Spike glycoprotein Receptor-binding domain, S glyc
• Note: For research use only
• Application notes: 1. Require BSL-2 or higher working environment 2. 5 μL per application (96-well plate) with at least 0.3 X 106 RLU / well. 3. Avoid freezing and thawing cycle to ensure the titer of the pseudovirus. 4. This product is for Research Use only5. Neutralization assay protocol is available
• Expiration Date: 12 months from date of receipt.

Neutralization Assay of Omicron BA.5 Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike Antibody, Neutralization antibodies: Anti-SARS-CoV-2 Spike antibodies, dilution: 0.03-1000 ng/mL. Pseudovirus: SARS-CoV-2 Omicron BA.5 Spike Pseudovirus, orb1671376. Infected Cells: ACE2-overexpressing 293T cells. Diluted antibody was incubated with Omicron BA.5 pseudovirus at 37 °C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 °C for 72 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.