SARS-CoV-2 Omicron BA.2 Variant Pseudovirus

• Catalog Number: orb1227401
• Category: Small Molecules
• Description: SARS-CoV-2 Omicron BA.2 Variant Pseudovirus
• Tested applications: NeA
• Form/Appearance: Liquid
• Target: S
• UniProt ID: P0DTC1
• Protein Sequence: Omicron Variant Spike BA.2 Met 1 - Cys 1251 (T19I, LPP24-26del, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K)
• Source: HEK293 cells
• NCBI: QHD43416
• Storage: The pseudovirus is stable in a liquid state at -70˚ for 12 months. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: DMEM/10% FBS
• Alternative names: Spike glycoprotein Receptor-binding domain, S glyc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Neutralization Assay of SARSCoV-2 Omicron BA.2 Pseudovirus by SARS-CoV-2 Omicron Spike Antibody. Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, dilution: 3-30, 000 ng/mL. Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, orb1227401. Infected Cells: ACE2-overexpressing 293T cells. Diluted antibody was incubated with Omicron BA.2 pseudovirus at 37 °C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 °C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.