SARS-CoV-2 Nucleocapsid Antibody

• Catalog Number: orb1274616
• Category: Antibodies
• Description: SARS-CoV-2 Nucleocapsid Antibody
• Clonality: Recombinant
• Clone Number: 1C7C7
• Tested applications: ELISA, IHC, WB
• Reactivity: Virus
• Isotype: Mouse IgG2a
• Immunogen: Recombinant nucleoprotein of SARS-CoV (severe acute respiratory syndrome).The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2.
• Concentration: > 1.0 mg/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8˚ C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80˚ C. Avoid Repeated Freeze Thaw Cycles.
• Buffer/Preservatives: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
• Alternative names: SARS-CoV-2 Nucleocapsid, SARS-CoV-2 Nucleoprotein,
• Note: For research use only
• Application notes: ELISA, IHC, WB (Quality Tested by BIorbyt); FC (Other applications as reported in literature)
• Expiration Date: 12 months from date of receipt.

Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15 min), blocked with 4% BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour).

Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane.

Exp1293 cells were transfected with expression plasmid coding for SARs2 NP and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supermatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.