SARS-CoV-2 Delta Variant Pseudovirus

• Catalog Number: orb1227400
• Category: Small Molecules
• Description: SARS-CoV-2 Delta Variant Pseudovirus
• Tested applications: NeA
• Form/Appearance: Liquid
• Target: S
• UniProt ID: P0DTC1
• Protein Sequence: Delta Variant Spike Met 1 - Cys 1252 (T19R, T95I, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N)
• Source: HEK293 cells
• NCBI: QHD43416
• Storage: The pseudovirus is stable in a liquid state at -70˚ for 12 months. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: DMEM/10% FBS
• Alternative names: Spike glycoprotein Receptor-binding domain, S glyc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Neutralization Assay of SARS-CoV-2 Delta Pseudovirus by SARS-CoV-2 Delta Spike Antibody. Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, dilution: 3-30, 000 ng/mL. Pseudovirus: SARS-CoV-2 Delta Spike Pseudovirus, orb1227400. Infected Cells: ACE2-overexpressing 293T cells. Diluted antibody was incubated with Delta pseudovirus at 37 °C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 °C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.