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Catalog Number | orb1945955 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 Delta Plus Variant Recombinant Spike Trimer His Tag |
Tested applications | ELISA |
Reactivity | Other |
Tag | His tag |
Isotype | SARS-CoV-2 |
Concentration | 0.5 mg/ml |
Dilution range | Control antigen for SARS-CoV-2 (COVID-19) diagnostic assays. Contains C-terminal His tag used for purification. |
Storage | 2-8°C |
Buffer/Preservatives | 1X PBS, 0.025% NaN3 |
Alternative names | Severe acute respiratory syndrome coronavirus 2 sp Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fcg fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.
SARS-CoV-2 spike protein variant purity by SDS-PAGE. Separation of SARS-CoV-2 spike recombinant protein (8xHis-Tag, 2 ug) reduced (R) and non-reduced (NR) condition followed by Coommassie blue staining shows >90% purity.
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