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SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD Protein (His & Avi), Biotinylated
Catalog Number: orb1953384
| Catalog Number | orb1953384 |
|---|---|
| Category | Proteins |
| Description | SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 28.49 kDa and the accession number is YP_009724390.1. |
| Tag | C-His-Avi |
| Purity | 98.00% |
| UniProt ID | YP_009724390.1 |
| MW | 28.49 kDa (predicted); 38.44 kDa (reducing condition, due to glycosylation) |
| Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Expression System | HEK293 Cells |
| Biological Origin | SARS-CoV-2 |
| Biological Activity | SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 28.49 kDa and the accession number is YP_009724390.1. |
| Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.4/BA.5/BA.5.2). Predicted N terminal: Arg 319 |
| Storage | -20°C |
| Note | For research use only |
