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SARS-CoV-2 (B.1.617.2) Spike S1+S2 trimer Protein (His & Avi), Biotinylated
Catalog Number: orb1953475
| Catalog Number | orb1953475 |
|---|---|
| Category | Proteins |
| Description | SARS-CoV-2 (B.1.617.2) Spike S1+S2 trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 138.03 kDa and the accession number is YP_009724390.1. |
| Tag | C-His-Avi |
| Purity | 98.00% |
| UniProt ID | YP_009724390.1 |
| MW | 138.03 kDa (predicted) |
| Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Expression System | HEK293 Cells |
| Biological Origin | SARS-CoV-2 |
| Biological Activity | SARS-CoV-2 (B.1.617.2) Spike S1+S2 trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 138.03 kDa and the accession number is YP_009724390.1. |
| Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations T19R, 156-157 deletion, R158D, L452R, T478K, D614G,F817P, A892P, A899P,A942P, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India. Predicted N terminal: Val 16 |
| Storage | -20°C |
| Note | For research use only |
