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Catalog Number | orb1953504 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76.48 kDa and the accession number is A0A6G7K2L4. |
Tag | C-His |
Purity | 98.00% |
MW | 76.48 kDa (predicted); 99.56 kDa (reducing condition, due to glycosylation) |
UniProt ID | A0A6G7K2L4 |
Expression System | HEK293 Cells |
Biological Origin | SARS-CoV-2 |
Biological Activity | SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76.48 kDa and the accession number is A0A6G7K2L4. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations A67V, HV69-70 deletion, T95I, G142D, VYY143-145 deletion, N211 deletion, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529) which emerged in the South Africa. Predicted N terminal: Val 16 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
> 90% as determined by SDS-PAGE | |
150-180 kDa |
98.00% | |
33.68 kDa (predicted); 54.37 kDa (reducing condition, due to glycosylation) |
98.00% | |
134.54 kDa (predicted) |
98.00% | |
77.94 kDa (predicted). Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |