SARS-CoV-2 (2019-nCoV) Spike Neutralizing Mouse Monoclonal Antibody

SKU: orb1048258

Description

SARS-CoV-2 (2019-nCoV) Spike Neutralizing Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ELISA
• Dilution range: ELISA=1:5000-10000
• Reactivity: Virus
• Predicted Reactivity: Virus

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 7G11
• Immunogen: Recombinant SARS-CoV-2 Spike S1 Protein (14-685/1213aa)
• Target: S
• Molecular Weight: 140 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Concentration: Lot Dependent
• Disclaimer: For research use only

Alternative Names

SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2.

Direct ELISA was used to detect the binding ability of anti-RBD monoclonal antibody to RBD domain proteins of different SARS-CoV-2 Mutant Strains. Immobilized SARS-CoV-2 RBD proteins, at 2 µg/ml (100 ul/Well) can bind Anti-RBD monoclonal antibody-HRP at 1 ug/ml (100 ul/Well).

The ACE2-coated plate is incubated with SARS-CoV-2 Spike RBD-HRP (WT) and Anti-SARS-CoV-2 Spike RBD Neutralizing antibody. Percent inhibition is calculated based on the OD value by inhibiting RBD: ACE2 interaction.

Quick Database Links

Gene Symbol

S