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Catalog Number | orb1520306 |
---|---|
Category | Antibodies |
Description | SARDH Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 868 and 918 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | SARDH |
UniProt ID | Q9UL12 |
NCBI | Q9UL12.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | BPR-2; SarDH; SARD; sarcosine dehydrogenase, mitoc Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse SARDH by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, K-562, LNCaP, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SARDH antibody (orb1520306) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human SARDH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SARDH antibody (orb1520306) used for IP at 2 µg per reaction.
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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