You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1524217 |
---|---|
Category | Antibodies |
Description | SARA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 1 and 50 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | SARA |
UniProt ID | O95405 |
NCBI | NP_004790.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | hSARA; protein phosphatase 1, regulatory subunit 1 Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human SARA by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SARA antibody orb1524217 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. SARA was also immunoprecipitated by rabbit anti-SARA antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
FC, IHC-P, WB | |
Bovine, Mouse, Porcine | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating