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Catalog Number | orb548252 |
---|---|
Category | Antibodies |
Description | SAE2 / UBA2 Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | AEAI-21 |
Tested applications | ICC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | Q9UBT2 |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of SAE2/UBA2 expression in A549 cell lysate.
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