S100G Antibody / Calbindin D9K

• Catalog Number: orb1822589
• Category: Antibodies
• Description: This gene encodes calbindin D9K, a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of calcium-binding proteins that includes calmodulin, parvalbumin, troponin C, and S100 protein. In the intestine, the protein is vitamin D-dependent and its expression correlates with calcium transport activity. The protein may increase Ca2+ absorption by buffering Ca2+ in the cytoplasm and increase ATP-dependent Ca2+ transport in duodenal basolateral membrane vesicles. [provided by RefSeq, Jul 2008]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Calbindin D9K antibody.
• UniProt ID: P29377
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: S100G/7516
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Aliquot the Calbindin D9K antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This Calbindin D9K antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human small intestine tissue with Calbindin D9K antibody (clone S100G/7516). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with Calbindin D9Kantibody (clone S100G/7516). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with Calbindin D9K antibody (clone S100G/7516). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Calbindin D9K antibody (clone S100G/7516) as confirmation of integrity and purity.