S100A9 Antibody

• Catalog Number: orb315186
• Category: Antibodies
• Description: S100A9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse Flow Cytometry, 1-3μg/1x106 cells, Mouse ELISA , 0.1-0.5μg/ml, Mouse, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 13049 MW
• UniProt ID: P31725
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein S100-A9;Calgranulin-B;Leukocyte L1 complex
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HEPA1-6 cells using anti-S100A9 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of S100A9 using anti-S100A9 antibody.Lane 1:mouse spleen tissue; 2:mouse lung tissue.

IF analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in immunocytochemical section of HEPA1-6 cells.

IHC analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in a paraffin-embedded section of rat spleen tissue.

IHC analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in a paraffin-embedded section of mouse spleen tissue.