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S100A9 Antibody / MRP14 / Calgranulin B / Calprotectin L1H

Catalog Number: orb2643763

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2643763
CategoryAntibodies
DescriptionRecognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer s cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
ClonalityRecombinant
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenAn affinity purified monocyte membrane preparation was used as the immunogen for this recombinant S100A9 antibody.
UniProt IDP06702
Tested applicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml
Application notesOptimal dilution of the antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberrMAC3781
Formula0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant S100A9 antibody is available for research use only.
NoteFor research use only
S100A9 Antibody / MRP14 / Calgranulin B / Calprotectin L1H

IHC staining of FFPE human spleen with recombinant S100A9 antibody (clone rMAC3781). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

S100A9 Antibody / MRP14 / Calgranulin B / Calprotectin L1H

IHC staining of FFPE human spleen with recombinant S100A9 antibody (clone rMAC3781). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

S100A9 Antibody / MRP14 / Calgranulin B / Calprotectin L1H

SDS-PAGE analysis of purified, BSA-free recombinant S100A9 antibody (clone rMAC3781) as confirmation of integrity and purity.