S100A8+A9 Complex Mouse Monoclonal Antibody

SKU: orb1040890

Description

S100A8+A9 Complex Mouse Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-200, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 10D11
• Immunogen: Recombinant human human S100A8+A9 Complex
• Target: S100A8
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; S100-A8; B8Ag; S10A8_HUMAN; S100A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen (CFAG); Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8 (MRP-8 | p8); S100 calcium-binding protein A8; Urinary stone protein band A; S10A8_MOUSE; Chemotactic cytokine CP-10; Pro-inflammatory S100 cytokine;

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (orb1040890) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (orb1040890) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (orb1040890) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant human S100-A8/A9 protein, His, Primary: Anti-S100A8+A9 Complex (orb1040890) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 34 kDa.

Quick Database Links

Gene Symbol

S100A8

UniProt

P05109