Rubicon Antibody

• Catalog Number: orb1526396
• Category: Antibodies
• Description: Rubicon Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 922 and 972
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Rubicon
• UniProt ID: Q92622
• NCBI: NP_055502.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RUN domain and cysteine-rich domain containing, Be
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Rubicon by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody orb1526396 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Rubicon by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rubicon antibody orb1526396 used for IP at 6 µg per reaction.