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Catalog Number | orb344611 |
---|---|
Category | Antibodies |
Description | Rub1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Yeast |
Isotype | IgG |
Immunogen | Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Lyophilized |
Purity | Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. |
Conjugation | Unconjugated |
UniProt ID | Q03919 |
NCBI | Q03919.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Rub1 antibody, NEDD8-like protein RUB- Read more... |
Note | For research use only |
Application notes | Rub1 Antibody is tested in western blot and defects a 6 kDa band corresponding to yeast Rub1. Most yeast cell lysates can be used as a positive control without induction or stimulation. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of against a yeast cell lysate using Rub1 antibody
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